| 1 | Overload of centrifugal Ultra-filtration device may cause partial sample lost | Common mistakes |
| 2 | Homemade Bradford reagent 5X | Simple solutions for lab life |
| 3 | Homemade E.coli chemically competent cells | Molecular biology |
| 4 | Replacement of Ethidium bromide with a safe stain for agarose gel | Gene Cloning |
| 5 | Safe Comassie blue staining for SDS page (compatible with microwave coloration) | Analitics |
| 6 | Rapid protein Expression and Solubility check using a 24 tip-horn sonicator | Protein purification |
| 7 | Small scale IMAC purification from Expi293 or Expi-CHO culture surnatant | Protein purification |
| 8 | ExpiCHO a powerful cell line for transient expression of biologics | Mab production |
| 9 | Not use low purity imidazole in buffer preparation for IMAC purification | Protein Purification |
| 10 | Use of 2F-Peracetyl-Fucose to produce low fucosilated mabs with ExpiCHO | Mab production |
| 11 | Chemical_enzymatic Cell lysis in high-throughput small scale protein purification from E.coli | Protein Purification |
| 12 | Insertion/deletion/replacement of N-terminal signal peptide sequences using PIPE cloning | Gene cloning |
| 13 | Densitometric Protein quantification from SDS-page using the ImageJ free software package | Analitics |
| 14 | Propagation and recombinant protein expression using ExpiCHO and Expi293 (WT and GNTI-) | Recombinant protein expression |
| 15 | An alternative way to fix the sticky pad to the Eppendorf S41i CO2 shakers | Simple solutions for lab life |
| 16 | Loading small sample volumes on AKTA instruments | Analitics |
| 17 | Alternative cheaper trasfection agents for recombinant antigen expression with Expi293 | Recombinant protein expression |
| 18 | MabSelect@ a cheaper resin for gravity flow purification of recombinant mabs | Mab purification |
| 19 | Pay attention to reverse GOI insertion in TOPO cloning | Gene cloning |
| 20 | Micro PEG solubility screening, a simple Tool for Biologics Design and Formulation | Mabs characterization |
| 21 | Chromas a free tool to check your Sanger sequencing results | Gene cloning |
| 22 | Tips for cleaning the OMNISEC RALS/LALS flow cell if get dirty! | Analitics |
| 23 | Selection of the right post-column filter to detect protein/antibody aggregates in LALS | Analitics |
| 24 | Rapid fluorimetric DNA plasmid quantification on 96 welll plate | Analitics |
| 25 | Choice of the right material to perform DNA and/or protein UV Spectrophotometric quantifications | Analitics |
| 26 | Guidelines to download a Video from blogger | ——- |
| 27 | DSF an useful tool for Monoclonal antibodies optimization and developabiltiy assesment | Mab characterization |
| 28 | Extracellular vesicle production using ExpiCHO | Cell biology |
| 29 | Simple solutions to improve laboratory routine work with few euro/dollars: #1 IKEA | Simple solution for lab life |
| 30 | How improve Specific heavy-light chain pairing in the production of bispecific mabs | Antibody engeeniring |
| 31 | Modern Approaches to modulate the antibody effector function | Antibody engeeniring |
| 32 | HemA, a selection marker for antibiotic free plasmid mantainance in E.coli | Recombinant protein expression |
| 33 | What about specificity of antibody isotyping kits? | mabs characterization |
