This section offers a collection of links to the answers I’ve personally shared on ResearchGate over the past 15 years.
I hope that you will found it usefull!
Click on the question to be re-directed to the answer
What exactly “BL21 Gold” cells are?
(Topic: Recombinant protein Expression)
Why competent cells (E.coli DH5alpha) cannot growth after trasfromation?
(Topic: Gene cloning – Molecular biology)
How do we select the right buffer pH on the basis of the protein pI?
(Topic: Protein Purification and Formulation)
How many time we can use the same Ni-NTA for protein purification?
(Topic: Protein Purification and Formulation)
Which is the minimun protein concentration required for SEC chromatography?
(Topic: Protein Purification and Formulation)
Yellow colour eluted from protein A antibody purification from Expi-CHO) whats happen?
(Topic: Protein Purification and Formulation)
How I do concentrate liters of cell medium?
(Topic: Cell culture)
Which are the differences between Tris-acetate and Tris tricine gels?
(Topic: Analytics)
Can i use SDS-page to detect S-S bonds?
(Topic: Analytics)
Why do we use Agarose gel for DNA and SDS-PAGE gel for protein?
(Topic: Analytics)
How to run guanidine hydrocloride sample in SDS-page?
(Topic: Analytics)