Hand on Protocools Index

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ProteoCool n°	Title	Topics
1	Cloning methods overview	Moleculal Biology
2	Primer Design: Restriction/Ligation	Molecular Biology
3	Primer Design: PIPE Cloning	Molecular Biology
4	PCR Colony screening	Molecular Biology
5	E.coli high cell density media overview	Recombinant protein expression
6	Rapid SDS-page coloration/decoloration using microwave	Simple solution for lab life
7	High cell density E.coli protein expression using EnpressoB media	Recombinant protein expression
8	Buffer Exchange methods overview	Biochemistry
9	2 Common mistakes causing sample lost using the AKTA Systems	Common mistakes
10	PARAFILM a simple support for sample loading on agarose gel	Simple solution for lab life
11	Rapid buffer <–> Exchange by PD-10	BIochemistry
12	EZNA HP MINI KIT II: MIDI KIT YIELDS from a MINI KIT format	Useful Tools and/or reagents
13	Subctractive IMAC	Protein purification
14	Endotrap: A simple and efficient tool for endotoxin removal	Protein purification
15	Overview on SDS-page	Protein/Antibody characterization
16	3 common mistakes in buffer preparation for protein purification	Common mistakes
17	Overview on simple bioinformatic tools for DNA sequence analysis	Useful Tools and/or reagents
18	Simple PCR test for Mycoplasma detection	Microbiology
19	Simple bioinformatic Tools for protein sequence analysis	Useful Tools and/or reagents
20	FAST READ 102: A multi-chamber device to speed up manual cell count	Useful Tools and/or reagents
21	3 common mistakes in E.coli culture	Common mistakes
22	Overview on tools for recombinant protein expression in E.coli	Recombinant Protein Expression
23	Taq Nova Red Taq	Useful Tools and/or reagents
24	DSF a simple and powerful tool for protein QC	Protein/Antibody characterization
25	Simple IMAC regeneration	Simple solution for lab life
26	Single channel pipette cleaning	Simple solution for lab life
27	Overview on Agarose Gel	Molecular Biology
28	Zinc: An alternative cheap and healthy metal for IMAC	Protein Purification
29	Expi293: A simple and powerfull mammalian expression system	Recombinant protein expression
30	Uniform 15N, 13C and 2H protein labeling using E.coliand more	Recombinant protein expression
31	Homemade Handrub Sanitizer	Simple solution for lab life
32	High cell density E.coli protein expression using YGR media	Recombinant protein expression
33	TIPS to avoid common mistakes in Spettroptotometry/Fluorimetry/Luminometry	Common mistakes
34	Inappropriate use of Ultrafiltration  for membrane protein  concentration	Common mistakes
35	Wrong AMPICILLIN concentration for transformation of BL21(DE3) deltaTolR E.coli	Common mistakes
36	2 component Epoxy adhesive to ready fix broken plastic laboratory equipment	Simple solution for lab life
37	Densitometric analisys of SDS-page using Image LAB software (license free version)	Useful Tools and/or reagent